Short-Term Chilled Storage of Zebrafish (Danio rerio) Embryos in Cryoprotectant As an Alternative to Cryopreservation
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منابع مشابه
Title: 1 SHORT TERM CHILLED STORAGE OF ZEBRAFISH (Danio rerio) EMBRYOS IN 2 CRYOPROTECTANT AS AN ALTERNATIVE TO CRYOPRESERVATION
23 As zebrafish embryos never been cryopreserved, we developed a protocol to store zebrafish embryos 24 (50% epiboly5.3 hpf) for up to 18 h at 0° C. Initial experiments to optimise cryoprotectant solution 25 demonstrated improved embryo hatching rate following chilling at 0°C for 18 h with 1 M MeOH + 26 0.1 M sucrose (56 ± 5%) compared to other combination of methanol (0.2-, 0.5 M) and sucrose ...
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ژورنال
عنوان ژورنال: Zebrafish
سال: 2015
ISSN: 1545-8547,1557-8542
DOI: 10.1089/zeb.2013.0961